Application of Scanning Electron Microscope in Palynology Study of Floral Resources By Indo-Malayan Stingless Bees Genus Tetragonula

Zubaidah A.H, A.R Shamsul Bahri, Junedah Sanusi, Nur Azura Adam, Azura A., Hamzah A.H.

  • Zubaidah Abu Hassan

Abstract

Application of Scanning Electron Microscope in Palynology Study of Floral Resources By Indo-Malayan Stingless Bees Genus Tetragonula

 

Zubaidah A.H¹, A.R Shamsul Bahri², Junedah Sanusi³, Nur Azura Adam4,

Azura A.5, Hamzah A.H.5

 

1 Department of Medical Microbiology, Faculty of Medicine, University of Malaya

2School of Science and food Technology, Universiti Malaysia Terengganu, 3Faculty of Agriculture, Universiti Putra Malaysia, 4Department of Anatomy, Faculty of Medicine, University of Malaya.
5 Electron Microscope Unit, Faculty of Medicine, University of Malaya

___________________________________________________________________________

Abstract

Palynology or study of pollen (pollen analysis) allows the identification of floral sources of stingless bees. The palynology (pollen analysis) was carried out on pollens grains foraged by the Indo-Malayan stingless bees. The objective of this study is to identifiy the flower preferred by the Tetragonula in their surrounding habitat.  The pollens collected by eight species of Tetragonula and their referrences flower were investigated using scanning electron microscope (SEM) with some modification on the SEM parameters to suit the samples. Pollen’s unique morphology is like fingerprint which can provide a basis for the identification of plant species.  About twenty-three (23) pollens have been observed and identified. The predominant pollens were Antigonon leptopus (Polygonaceae) and Elaeis guineensis (Arecaceae) with each collected by about four (4) species of stingless bees; followed by Averrhoa carambola (Oxalidaceae), Capsicum annuum (Solanaceae), Citrus microcarpa (Rutaceae),  Cocos nucifera (Arecaceae) and Sphagneticola tribolata (Asteraceae) with each collected by about three (3) species of stingless bees. Tetragonula fuscobalteata was found as the most generalist foragers which had collected almost twelve (12) type of pollens, followed by Tetragonula laeviceps with nine (9) species of pollens. It can be concluded the identification of pollens and stingless bee-collected pollens based on morphological ultrastructural detail using SEM can provide the valuable and specific information of pollens and plant-insect interaction.

Keywords: pollen, Tetragonula, Scanning Electron Microscope

___________________________________________________________________________

ARTICLE INFO

Received 15th November 2018

Received in revised form 30th November 2018

Accepted 1st December 2018

*Corresponding author: Zubaidah Abu Hassan

Tel: +60163235584 e mail: zubae@um.edu.my

Copyright Malaysian Journal of Microscopy (2018). All rights reserved. ISSN: 1823-7010 eISSN 2600-7444

Published
2018-12-31
Section
Original Research Articles