Molecular characterization of bacteria isolated from the skin of cantaloupe fruit using 16S ribosomal RNA gene sequence analysis

Musa, A. A. *, Saif Asheyab, A. A., Shehu, I., Chong, C. S., Nafi’u, S. A. and Abdulmumin, Y.

  • Musa Ahmed Abubakar

Abstract

Molecular characterization of bacteria isolated from the skin of cantaloupe fruit using 16S ribosomal RNA gene sequence analysis

Musa, A. A. 1*, Saif Asheyab, A. A.2, Shehu, I.3, Chong, C. S.4, Nafi’u, S. A.1 and Abdulmumin, Y.5

1Department of Science Laboratory Technology, Kano State Polytechnic, Kano, Nigeria

2Department of Applied Biological Sciences & Arts, Jordan University of Science & Technology Irbid, Jordan

3Department of Microbiology, Kaduna State University, Kaduna, Nigeria

4Department of Biotechnology, Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia (UTM)

5Department of Biochemistry, Kano University of Science & Technology Wudil, Kano, Nigeria

 

Abstract

Fresh cut fruit and vegetable industry is one of the fastest developing food industries in the world. The present study was conducted with the aim to isolate bacteria from cantaloupe fruit skin sample collected from cut fruit industry in Johor Baharu, Malaysia and to identify the bacteria using 16S ribosomal (rRNA) gene analysis. Four unique bacterial strains were isolated and distinguished based on their morphological appearance through culture and microscopy analysis. For bacterial identification, the PCR products of the 16S rRNA gene were amplified, purified and sent for sequencing to 1st Base Sdn. Bhd Malaysia. The sequence data were then compared with other gene sequences in GenBank database (NCBI) using a BLAST search to find closely related sequences. The isolated bacteria were identified as Exiguobacteriumsp. (CG1), Exiguobacteriumsp. (CG2), Pseudomonassp. (CG3), and Microbacteriumsp. (CG4) after sequencing. Therefore, the association of fresh cut fruits with these bacteria can possibly cause contamination which might lead to outbreaks of human disease. Thus, proper cut fruits processing methods that could inhibit the growth or kill these bacteria are important to ensure the safety of cantaloupe cut fruits.

 

Keywords:Cantaloupe fruit; bacterial isolate, 16S rRNA sequence analysis, phylogenetic analysis.

ARTICLE INFO

Received 7th November 2018

Received in revised form 22nd November 2018

Accepted 23rd November 2018

*Corresponding author: Dr. Musa Ahmed Abubakar

Tel: +2348061619400e-mail: aamusadiso@yahoo.com

Copyright Malaysian Journal of Microscopy (2018). All rights reserved. ISSN: 1823-7010 eISSN 2600-7444

Published
2018-12-31
Section
Original Research Articles