The protective effects of Stachytarpheta jamaicensis (L.) Vahl on LPS-induced hepatic and renal injury in ICR mice

Thiagarajan, S. K., Mahadi, I. H., Jamaluddin, N., Yong Y. K., Liew, P. M., Mat Taib, C. N., Hussain, M. K., Perumal, K. V., Bahari, H.*

  • Hasnah Bahari

Abstract

The protective effects of Stachytarpheta jamaicensis (L.) Vahl on LPS-induced hepatic and renal injury in ICR mice

 

Thiagarajan, S. K.1, Mahadi, I. H.2, Jamaluddin, N.2, Yong Y. K.1, Liew, P. M.1, Mat Taib, C. N.1, Hussain, M. K.2, Perumal, K. V.1, Bahari, H.1*

 

1Department of Human Anatomy, 2Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Selangor, Malaysia

 

 

Abstract

Stachytarpheta jamaicensis (SJ) is one of the plants that being used as alternative in traditional treatments that possess medicinal properties. SJ has been found to exhibit anti-inflammatory activity in acute and chronic models of inflammation, and thus protects the liver. However, its effect on liver and kidney injury induced by lipopolysaccharide remains unclear. To examine the protective effects of SJ on liver and kidney of LPS-induced endotoxemia in ICR mice via determination of lipid peroxidation and histology assessment. A total of 18 male ICR mice (20-30g, 6-8 weeks) were divided into six groups. Control (CTR) and negative control (NEG) groups received normal saline, while treatment groups received dexamethasone (DEX; 5 mg/kg bodyweight (BW)), and ethyl acid extract of SJ (10,100, and 150mg/kg BW) respectively, for seven days consecutively. On day 8, all groups (except control group) were injected with lipopolysaccharides (LPS; 1mg/kg BW) intradermally. After 1 hour of LPS injection, the mouse were euthanized and livers were excised, weighed and kept for assessment of  thiobarbituric acid reactive substances-malondialdehyde (TBARs-MDA) assay and histology assessment by haematoxylin-eosin staining. The SJ supplementation slightly decreased the lipid peroxidation in liver as compared to NEG group. In histology observation, the higher dosage of SJ at 150mg/kg BW improved the structure of hepatocytes and less congestion of central vein. In lesion score of the liver, SJ150 has significantly lower of lesion score compared to NEG. Whereas, in kidney tissue, the supplementation of SJ at 100 and 150 mg/kg BW significantly decreases lipid peroxidation level compared to NEG. In kidney lesion score, SJ150 was significantly lower by 55.32% compared to NEG. Overall, supplementation of SJ in LPS-induced endotoxemia in ICR mice may have potential in lowering lipid peroxidation and preventing liver and kidney injury. Further assessment should be done to explore the mechanisms of SJ in preventing the inflammatory mediators induced by LPS that leads to liver and kidney injury.

 

Keywords: Lipopolysaccharide; Stachytarpheta jamaicensis; Lipid peroxidation; Liver; Kidney

 

ARTICLE INFO

Received 31st August 2018

Received in revised form 7th November 2018

Accepted 12th November 2018

*Corresponding author: Dr. Hasnah Bahari

Tel: +603 8947 2769 e-mail: haba@upm.edu.my

Copyright Malaysian Journal of Microscopy (2018). All rights reserved. ISSN: 1823-7010 eISSN 2600-7444

Published
2018-12-31
Section
Original Research Articles